Five children with 45,X mosaicism have been born to mothers with 45,X mosaicism all had a 46,XX cell line as well. Two females are described, mother and daughter, who had the Turner phenotype and spontaneous sexual development. Six women with a 45, X chromosome complement are known to have delivered normal infants with no chromosomal abnormality. The analysis of Barr bodies in cell nuclei from easily accessible tissues such as buccal mucosa, hair root and blood cells allows provisional description of the sex chromosome status of individuals hermaphroditism, gonadal, and some complicated anomalies, whereas the use of amniotic fluid assists a prenatal sex diagnosis. Her menstrual cycles are irregular and, most probably, anovulatory.
To our surprise, Y-chromatin was observed in 96 of the nuclei in smears from the uncultured gonad. Cultured fibroblasts from the right gonad had also a 46,XX complement.
The mean percentage of sex chromatin in cases of fibroadenoma 54.91☖.06 was found to be similar to control cases 54.6☖.
#Chromatin negative nuclei buccal smear skin#
Her buccal smear was X-chromatin negative and karyotypes from peripheral blood lymphocytes and skin fibroblasts showed a 45,X chromosome constitution. An ambisexual male infant showed a normal female karyotype by the leukocyteculture technique in two different occasions. Also the sex chromatin counts in the buccal smears and peripheral blood films prepared from the patients of fibroadenoma breast and carcinoma breast were compared. The daughter is short and had spontaneous sexual development, including menstruation at the age of 15 years. She had a successful uncomplicated pregnancy at the age of 25 years. Clusters of cells with scant cytoplasm and dark nuclei represent the morphological. The mother is short and had ovulatory menstrual cycles, normal breast development, X-chromatin negative buccal smear, 45,X chromosomal pattern in her peripheral blood lymphocytes, and 45,X/46,X,r(X) mosaicism in her skin, with the majority of the cells (85%) showing X monosomy. Dense hyperchromatic cell groups are considered common diagnostic problems in cytopathological evaluations. (from King & Stansfield, A Dictionary of Genetics, 4th ed)Ī structure found in a female mammalian cell containing an unpaired X chromosome that has become densely heterochromatic, silenced and localized at the nuclear periphery.Two females are described, mother and daughter, who had the Turner phenotype and spontaneous sexual development. Each X CHROMOSOME, in excess of one, forms sex chromatin (Barr body) in the mammalian nucleus. Further sex chromatin preparations were obtained from skin culture. Sex chromatin bodies are found by this observer (A.M.B.)withafrequencyof25-60innormalfemales and are not observed in normal males. In the interphase nucleus, a condensed mass of chromatin representing an inactivated X chromosome. the I20 nuclei examined in buccal smear preparations, 6 (5)hada single, normal-sized, sex chromatin body. The inactive, condensed X chromosome in a female somatic cell. On buccal smear, X-chromatin negative nuclei wererevealed. One hundred and ninety-nine blood samples were collected from scavenging teenagers in Alaba International market (n 95) and the control group (n 104). Except for mild anaemia, a raised ESRandleucocytosis, thelaboratoryinves. Chromatin that is part of a sex chromosome. This study investigated the levels of Pb, Ni, Cd, and Cr in the blood, and DNA damage in exfoliated buccal cavity of scavenging teenagers at Alaba International electronic market, Nigeria.